p irs 1 ser307 Search Results


phosphor irs1 ser307  (Novus Biologicals)
90
Novus Biologicals phosphor irs1 ser307
Figure 1. Insulin signaling pathway was impaired in hGLs from patients with PCOS with IR. Insulin-stimulated IR-b Tyr1150/1151 phosphorylation (A, B), <t>IRS1</t> <t>Ser307</t> phosphorylation (A, C), total IRS2 expression (A, D), IRS1/2 Tyr612 phosphorylation (A, E), Akt Ser473 phosphorylation (A, F), and glucose uptake (A, G) in hGLs from either patients without PCOS without IR (n = 7) or with IR (n = 7) and either patients with PCOS without IR (n = 7) or with IR (n = 7). All values are means 6 SEM. *P , 0.05, **P , 0.01, against insulin in non-PCOS without IR or PCOS with IR group; &P , 0.05, against insulin in non-PCOS with IR group; #P , 0.05, ##P , 0.01, against insulin in PCOS without IR group.
Phosphor Irs1 Ser307, supplied by Novus Biologicals, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/p+irs+1+ser307/pm31311314-66-82-87?v=Novus+Biologicals
Average 90 stars, based on 1 article reviews
phosphor irs1 ser307 - by Bioz Stars, 2026-07
90/100 stars
  Buy from Supplier
rat pirs1 elisa kit  (MyBiosource Biotechnology)
90
MyBiosource Biotechnology rat pirs1 elisa kit
Figure 1. Insulin signaling pathway was impaired in hGLs from patients with PCOS with IR. Insulin-stimulated IR-b Tyr1150/1151 phosphorylation (A, B), <t>IRS1</t> <t>Ser307</t> phosphorylation (A, C), total IRS2 expression (A, D), IRS1/2 Tyr612 phosphorylation (A, E), Akt Ser473 phosphorylation (A, F), and glucose uptake (A, G) in hGLs from either patients without PCOS without IR (n = 7) or with IR (n = 7) and either patients with PCOS without IR (n = 7) or with IR (n = 7). All values are means 6 SEM. *P , 0.05, **P , 0.01, against insulin in non-PCOS without IR or PCOS with IR group; &P , 0.05, against insulin in non-PCOS with IR group; #P , 0.05, ##P , 0.01, against insulin in PCOS without IR group.
Rat Pirs1 Elisa Kit, supplied by MyBiosource Biotechnology, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/p+irs+1+ser307/pm37820996-46-27-32?v=MyBiosource+Biotechnology
Average 90 stars, based on 1 article reviews
rat pirs1 elisa kit - by Bioz Stars, 2026-07
90/100 stars
  Buy from Supplier
anti-p-irs-1 (ser307)  (ZenBio)
90
ZenBio anti-p-irs-1 (ser307)
Figure 1. Insulin signaling pathway was impaired in hGLs from patients with PCOS with IR. Insulin-stimulated IR-b Tyr1150/1151 phosphorylation (A, B), <t>IRS1</t> <t>Ser307</t> phosphorylation (A, C), total IRS2 expression (A, D), IRS1/2 Tyr612 phosphorylation (A, E), Akt Ser473 phosphorylation (A, F), and glucose uptake (A, G) in hGLs from either patients without PCOS without IR (n = 7) or with IR (n = 7) and either patients with PCOS without IR (n = 7) or with IR (n = 7). All values are means 6 SEM. *P , 0.05, **P , 0.01, against insulin in non-PCOS without IR or PCOS with IR group; &P , 0.05, against insulin in non-PCOS with IR group; #P , 0.05, ##P , 0.01, against insulin in PCOS without IR group.
Anti P Irs 1 (Ser307), supplied by ZenBio, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/p+irs+1+ser307/pmc08034456-89-35-38?v=ZenBio
Average 90 stars, based on 1 article reviews
anti-p-irs-1 (ser307) - by Bioz Stars, 2026-07
90/100 stars
  Buy from Supplier
irs1 [p ser307] antibody  (Bio-Techne corporation)
90
Bio-Techne corporation irs1 [p ser307] antibody
Figure 1. Insulin signaling pathway was impaired in hGLs from patients with PCOS with IR. Insulin-stimulated IR-b Tyr1150/1151 phosphorylation (A, B), <t>IRS1</t> <t>Ser307</t> phosphorylation (A, C), total IRS2 expression (A, D), IRS1/2 Tyr612 phosphorylation (A, E), Akt Ser473 phosphorylation (A, F), and glucose uptake (A, G) in hGLs from either patients without PCOS without IR (n = 7) or with IR (n = 7) and either patients with PCOS without IR (n = 7) or with IR (n = 7). All values are means 6 SEM. *P , 0.05, **P , 0.01, against insulin in non-PCOS without IR or PCOS with IR group; &P , 0.05, against insulin in non-PCOS with IR group; #P , 0.05, ##P , 0.01, against insulin in PCOS without IR group.
Irs1 [P Ser307] Antibody, supplied by Bio-Techne corporation, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/p+irs+1+ser307/bio-techne+corporation___nbp1-78078?v=Bio-Techne+corporation
Average 90 stars, based on 1 article reviews
irs1 [p ser307] antibody - by Bioz Stars, 2026-07
90/100 stars
  Buy from Supplier
irs-1(ser307) polyclonal antibody  (Bioss)
90
Bioss irs-1(ser307) polyclonal antibody
Figure 1. Insulin signaling pathway was impaired in hGLs from patients with PCOS with IR. Insulin-stimulated IR-b Tyr1150/1151 phosphorylation (A, B), <t>IRS1</t> <t>Ser307</t> phosphorylation (A, C), total IRS2 expression (A, D), IRS1/2 Tyr612 phosphorylation (A, E), Akt Ser473 phosphorylation (A, F), and glucose uptake (A, G) in hGLs from either patients without PCOS without IR (n = 7) or with IR (n = 7) and either patients with PCOS without IR (n = 7) or with IR (n = 7). All values are means 6 SEM. *P , 0.05, **P , 0.01, against insulin in non-PCOS without IR or PCOS with IR group; &P , 0.05, against insulin in non-PCOS with IR group; #P , 0.05, ##P , 0.01, against insulin in PCOS without IR group.
Irs 1(ser307) Polyclonal Antibody, supplied by Bioss, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/p+irs+1+ser307/bioss___bs-2736r?v=Bioss
Average 90 stars, based on 1 article reviews
irs-1(ser307) polyclonal antibody - by Bioz Stars, 2026-07
90/100 stars
  Buy from Supplier

Image Search Results


Figure 1. Insulin signaling pathway was impaired in hGLs from patients with PCOS with IR. Insulin-stimulated IR-b Tyr1150/1151 phosphorylation (A, B), IRS1 Ser307 phosphorylation (A, C), total IRS2 expression (A, D), IRS1/2 Tyr612 phosphorylation (A, E), Akt Ser473 phosphorylation (A, F), and glucose uptake (A, G) in hGLs from either patients without PCOS without IR (n = 7) or with IR (n = 7) and either patients with PCOS without IR (n = 7) or with IR (n = 7). All values are means 6 SEM. *P , 0.05, **P , 0.01, against insulin in non-PCOS without IR or PCOS with IR group; &P , 0.05, against insulin in non-PCOS with IR group; #P , 0.05, ##P , 0.01, against insulin in PCOS without IR group.

Journal: FASEB journal : official publication of the Federation of American Societies for Experimental Biology

Article Title: Elevated chemerin induces insulin resistance in human granulosa-lutein cells from polycystic ovary syndrome patients.

doi: 10.1096/fj.201802829R

Figure Lengend Snippet: Figure 1. Insulin signaling pathway was impaired in hGLs from patients with PCOS with IR. Insulin-stimulated IR-b Tyr1150/1151 phosphorylation (A, B), IRS1 Ser307 phosphorylation (A, C), total IRS2 expression (A, D), IRS1/2 Tyr612 phosphorylation (A, E), Akt Ser473 phosphorylation (A, F), and glucose uptake (A, G) in hGLs from either patients without PCOS without IR (n = 7) or with IR (n = 7) and either patients with PCOS without IR (n = 7) or with IR (n = 7). All values are means 6 SEM. *P , 0.05, **P , 0.01, against insulin in non-PCOS without IR or PCOS with IR group; &P , 0.05, against insulin in non-PCOS with IR group; #P , 0.05, ##P , 0.01, against insulin in PCOS without IR group.

Article Snippet: After determination of protein concentration by Bradford assay, 30 mg of protein from each sample was electrophoresed on a 10% SDS-polyacrylamide gel and transferred to a nitrocellulosemembrane.After blockingwith 5% nonfat milk, the membrane was incubated overnight at 4°C with antibodies against chemerin (1:1000 dilutions, AF2324; R&D Systems), CMKLR1 (1:500 dilutions, ab64881; Abcam), total insulin receptor b subunit (IR-b) (1:1000 dilutions, 3025; Cell Signaling Technology, Danvers, MA, USA), phosphorylated (phosphor)-IR (Tyr1150/1151) (1:1000 dilutions, 3024; Cell Signaling Technology), total IRS1 (1:500 dilutions, ab40777; Abcam), phosphor-IRS1 (Ser307) (1:500 dilutions, NB100-74448; Novus Biologicals), total IRS2 (1:1000 dilutions, 4502; Cell Signaling Technology), phosphor-IRS1/2 (Tyr612) (1:1000 dilutions, sc-17195-R; Santa Cruz Biotechnology, Dallas, TX, USA), total Akt (1:2000 dilutions, 4691; Cell Signaling Technology), phosphor-Akt (Ser473) (1:2000 dilutions, 4060; Cell Signaling Technology), and GLUT4 (1:2500 dilutions, ab654; Abcam).

Techniques: Phospho-proteomics, Expressing

Figure 3. Chemerin impaired insulin signaling and glucose uptake in cultured hGLs. A–E) Effect of prior chemerin (100 ng/ml, 24 h) treatment on the levels of IR-b Tyr1150/1151 phosphorylation (A, B), IRS1 Ser307 phosphorylation (A, C), IRS1/2 Tyr612 phosphorylation (A, D), and Akt Ser473 phosphorylation (A, E) (n = 5) after insulin (100 nM; 15 min) stimulation. F) Effect of prior chemerin (100 ng/ml, 24 h) treatment on total GLUT4 expression (n = 5). G–H) Effect of prior chemerin (100 ng/ml, 24 h) treatment on GLUT4 translocation (membrane GLUT4/cytoplasm GLUT4) (n = 5) and glucose uptake (n = 5) after insulin (100 nM, 30 min) stimulation. All values are means 6 SEM. *P , 0.05, **P , 0.01, ***P , 0.001, against control; #P , 0.05, ##P , 0.01, against +insulin.

Journal: FASEB journal : official publication of the Federation of American Societies for Experimental Biology

Article Title: Elevated chemerin induces insulin resistance in human granulosa-lutein cells from polycystic ovary syndrome patients.

doi: 10.1096/fj.201802829R

Figure Lengend Snippet: Figure 3. Chemerin impaired insulin signaling and glucose uptake in cultured hGLs. A–E) Effect of prior chemerin (100 ng/ml, 24 h) treatment on the levels of IR-b Tyr1150/1151 phosphorylation (A, B), IRS1 Ser307 phosphorylation (A, C), IRS1/2 Tyr612 phosphorylation (A, D), and Akt Ser473 phosphorylation (A, E) (n = 5) after insulin (100 nM; 15 min) stimulation. F) Effect of prior chemerin (100 ng/ml, 24 h) treatment on total GLUT4 expression (n = 5). G–H) Effect of prior chemerin (100 ng/ml, 24 h) treatment on GLUT4 translocation (membrane GLUT4/cytoplasm GLUT4) (n = 5) and glucose uptake (n = 5) after insulin (100 nM, 30 min) stimulation. All values are means 6 SEM. *P , 0.05, **P , 0.01, ***P , 0.001, against control; #P , 0.05, ##P , 0.01, against +insulin.

Article Snippet: After determination of protein concentration by Bradford assay, 30 mg of protein from each sample was electrophoresed on a 10% SDS-polyacrylamide gel and transferred to a nitrocellulosemembrane.After blockingwith 5% nonfat milk, the membrane was incubated overnight at 4°C with antibodies against chemerin (1:1000 dilutions, AF2324; R&D Systems), CMKLR1 (1:500 dilutions, ab64881; Abcam), total insulin receptor b subunit (IR-b) (1:1000 dilutions, 3025; Cell Signaling Technology, Danvers, MA, USA), phosphorylated (phosphor)-IR (Tyr1150/1151) (1:1000 dilutions, 3024; Cell Signaling Technology), total IRS1 (1:500 dilutions, ab40777; Abcam), phosphor-IRS1 (Ser307) (1:500 dilutions, NB100-74448; Novus Biologicals), total IRS2 (1:1000 dilutions, 4502; Cell Signaling Technology), phosphor-IRS1/2 (Tyr612) (1:1000 dilutions, sc-17195-R; Santa Cruz Biotechnology, Dallas, TX, USA), total Akt (1:2000 dilutions, 4691; Cell Signaling Technology), phosphor-Akt (Ser473) (1:2000 dilutions, 4060; Cell Signaling Technology), and GLUT4 (1:2500 dilutions, ab654; Abcam).

Techniques: Cell Culture, Phospho-proteomics, Expressing, Translocation Assay, Membrane, Control

Figure 4. Knockdown of CMKLR1 restored insulin sensitivity in chemerin-treated hGLs. A) Efficacy of siRNA-mediated knockdown of CMKLR1 in hGLs (n = 4). B–G) Effect of prior chemerin (100 ng/ml, 24 h) treatment on the induction of IRS1 Ser307 phosphorylation (B, C), IRS1/2 Tyr612 phosphorylation (B, D), Akt Ser473 phosphorylation (B, E), GLUT4 translocation (membrane GLUT4/cytoplasm GLUT4) (F) (n = 4), and glucose uptake (G) (n = 4) by insulin (100 nM) in the presence or absence of siRNA-mediated knockdown of CMKLR1. All values are means 6 SEM. *P , 0.05, **P , 0.01, ***P , 0.001, against negative control (NC) or +insulin; #P , 0.05, ##P , 0.01, against chemerin + insulin.

Journal: FASEB journal : official publication of the Federation of American Societies for Experimental Biology

Article Title: Elevated chemerin induces insulin resistance in human granulosa-lutein cells from polycystic ovary syndrome patients.

doi: 10.1096/fj.201802829R

Figure Lengend Snippet: Figure 4. Knockdown of CMKLR1 restored insulin sensitivity in chemerin-treated hGLs. A) Efficacy of siRNA-mediated knockdown of CMKLR1 in hGLs (n = 4). B–G) Effect of prior chemerin (100 ng/ml, 24 h) treatment on the induction of IRS1 Ser307 phosphorylation (B, C), IRS1/2 Tyr612 phosphorylation (B, D), Akt Ser473 phosphorylation (B, E), GLUT4 translocation (membrane GLUT4/cytoplasm GLUT4) (F) (n = 4), and glucose uptake (G) (n = 4) by insulin (100 nM) in the presence or absence of siRNA-mediated knockdown of CMKLR1. All values are means 6 SEM. *P , 0.05, **P , 0.01, ***P , 0.001, against negative control (NC) or +insulin; #P , 0.05, ##P , 0.01, against chemerin + insulin.

Article Snippet: After determination of protein concentration by Bradford assay, 30 mg of protein from each sample was electrophoresed on a 10% SDS-polyacrylamide gel and transferred to a nitrocellulosemembrane.After blockingwith 5% nonfat milk, the membrane was incubated overnight at 4°C with antibodies against chemerin (1:1000 dilutions, AF2324; R&D Systems), CMKLR1 (1:500 dilutions, ab64881; Abcam), total insulin receptor b subunit (IR-b) (1:1000 dilutions, 3025; Cell Signaling Technology, Danvers, MA, USA), phosphorylated (phosphor)-IR (Tyr1150/1151) (1:1000 dilutions, 3024; Cell Signaling Technology), total IRS1 (1:500 dilutions, ab40777; Abcam), phosphor-IRS1 (Ser307) (1:500 dilutions, NB100-74448; Novus Biologicals), total IRS2 (1:1000 dilutions, 4502; Cell Signaling Technology), phosphor-IRS1/2 (Tyr612) (1:1000 dilutions, sc-17195-R; Santa Cruz Biotechnology, Dallas, TX, USA), total Akt (1:2000 dilutions, 4691; Cell Signaling Technology), phosphor-Akt (Ser473) (1:2000 dilutions, 4060; Cell Signaling Technology), and GLUT4 (1:2500 dilutions, ab654; Abcam).

Techniques: Knockdown, Phospho-proteomics, Translocation Assay, Membrane, Negative Control